Study of Human Cytomegalovirus mRNA Transcription Profile at Different Infection Stages in Infants

VernacularTitle:婴幼儿人巨细胞病毒mRNA在不同感染时期表达的研究
Author: Juan LI ; Fenglin LIU ; Shuhong ZHANG
Publication Type:Journal Article
Keywords: cytomegalovirus; infant,newborn; IE mRNA; pp67 mRNA; virus identification
From: Tianjin Medical Journal 2014;(8):805-807
CountryChina
Language:Chinese
Abstract: Objective Human cytomegalovirus (HCMV) can induce infection in multiple organs, including genitouri-nary system, central nervous system and liver. It can even lead to serious defects and death. China, as a high-incidence area of HCMV, need employ early treatment. However, the sensitivity of present HCMV diagnosis is not satisfied. Methods In the present study, we focused on transcription level of HCMV IE and pp67 mRNA using real-time quantitative PCR. Results IE mRNA was positive even in a portion of patients who were regarded as negative by traditional DNA diagnosis method. On the other hand, pp67 mRNA that were normally expressed at the late stage of HCMV infection was 100%posi-tive in the first-visit patients who were regarded as positive using traditional DNA diagnosis method. Conclusion The pres-ent study indicated that mRNA detection based on PCR method was more sensitive compared with DNA detection for assess-ing HCMV virus infection. Finally, different treatment should be performed upon mRNA transcription profile at different in-fection stages.