Influence of simvastatin on differentiation, proliferation, migration and adhesion of smooth muscle progenitor cells
10.3969/j.issn.1008-0074.2014.05.13
- VernacularTitle:辛伐他汀对平滑肌祖细胞分化、增殖、迁移及黏附能力的影响
- Author:
Yanxia LIU
;
Lan HUANG
;
Xianyang ZHU
;
Po ZHANG
;
Duanzhen ZHANG
;
Xiumin HAN
;
Qiguang WANG
- Publication Type:Journal Article
- Keywords:
Simvastatin;
Myocytes,smooth muscle;
Cell differentiation;
Cell proliferation
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2014;23(5):519-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe influence of simvastatin on differentiation ,proliferation ,migration and adhesion of marrow-derived smooth muscle progenitor cells (SPCs) and screen coated eluting stent drugs of new generation . Methods :The mononuclear cells (MNCs) were isolated from rat marrow by density gradient centrifugation method , and then plated on fibronectin-coated culture dishes ,after culture 8d ,marrow-derived SPCs were identified by α-smooth muscle actin (α-SMA) immunofluorescent staining and counted under inverted fluorescence microscope .The MNCs and adhesion cells were treated with simvastatin (0.01~10 μmol/L) respectively for 8 d and 24h .SPCs pro-liferation ,migration and adhesion were observed by Tritium thymidine (3 H-TdR) intake method ,modified Boyden chamber assay and adhesion assay .Results:Compared with control group (no simvastatin intervention ) ,0.01μmol/L simvastatin significantly inhibited the MNCs differentiation towards SPCs [ (85 ± 4) vs .(79 ± 5)] ,proliferation [ (4070 ± 184) vs .(3833 ± 126)] ,migration [ (44 ± 3) vs .(39 ± 3)] and adhesion of SPCs [ (59 ± 5) vs .(52 ± 4)] , P<0.05 all ,and number of SPCs significantly reduced along with simvastatin concentration increased (P<0.01) . Conclusion:Simvastatin could inhibit the differentiation ,proliferation ,migration and adhesion of marrow-derived smooth muscle progenitor cells .
