Ultra-performance liquid chromatography electrospray ionization-tandem mass spectrometry method for the simultaneous determination of itraconazole and hydroxy itraconazole in human plasma
10.1016/j.jpha.2013.09.005
- Author:
Ashish DWIVEDI
;
Bhupinder SINGH
;
Sandeep SHARMA
;
R.s. LOKHANDAE
;
Naveen DUBEY
- Publication Type:Journal Article
- Keywords:
Itraconazole;
Hydroxy itraconazole;
Ultra-performance liquid chromatography;
Human plasma;
Simultaneous analysis
- From:
Journal of Pharmaceutical Analysis
2014;(5):316-324
- CountryChina
- Language:Chinese
-
Abstract:
A highly sensitive, selective, and precise ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for simultaneous quantification of itraconazole and hydroxy itraconazole in human plasma by a single liquid-liquid extraction step. The precursor to product ion transitions of m/z 705.3/392.3, m/z 721.2/408.3 and m/z 708.2/435.4 were used to detect and quantify itraconazole, hydroxy itraconazole and itraconazole-d3 respectively. The lower limit of quantitation was found to be 0.500 ng/mL for itraconazole and 1.00 ng/mL for hydroxy itraconazole. The mean recoveries for itraconazole and hydroxy itraconazole were found to be 100.045% and 100.021%, respectively. This developed method with a chromatographic run time of 2.0 min was successfully applied to a bioequivalence study of 100 mg itraconazole capsule.
