Simultaneous determination of three curcuminoids in Curcuma longa L. by high performance liquid chromatography coupled with electrochemical detection
10.1016/j.jpha.2013.10.002
- Author:
Yuling LONG
;
Wenpeng ZHANG
;
Fang WANG
;
Zilin CHEN
- Publication Type:Journal Article
- Keywords:
Curcumin;
High performance liquid chromatography;
Electrochemical detec-tion;
Curcuma longa L
- From:
Journal of Pharmaceutical Analysis
2014;(5):325-330
- CountryChina
- Language:Chinese
-
Abstract:
A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4-H3PO4 (pH 5.0) (50:50, v/v) was used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 0.227-114μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 ? 8 M, which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06%to 1.88%for intra-day precision and from 4.30%to 5.79%for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3%to 111%were obtained.
