The Effects ofβ-Sheet Breaker Peptide H102 on ERK Signal Transduction Pathway in Brain of PAP Double Transgenic Mice
10.3969/j.issn.0253-9896.2014.07.007
- VernacularTitle:β片层阻断肽H102对PAP小鼠脑内ERK信号转导通路的影响
- Author:
Bingyan WANG
;
Fengxian SUN
;
Laixiang LIN
;
Shumei XU
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
mitogen-activated protein kinase kinases;
mice,transgenic;
administration,intrana-sal;
ERK signal transduction pathway;
β-sheet breaker;
RAS;
P-MEK;
P-ERK
- From:
Tianjin Medical Journal
2014;(7):650-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the activation of β-sheet breaker peptide H102 on ERK signal transduction pathway in brain of PAP double transgenic mice. Methods PAP double transgenic mice were randomly divided into model group and H102 treatment group (n=10 for each group). A group of C57BL/6J mice with the same genetic background was served as controls. H102 (5.8 mg/kg) 5 μL was infused by intranasal administration to mice in H102 treatment group, and equal volume of blank solution of H102 (chitosan, BSA) was given to mice in control group and model group. The ability of spatial reference memory was tested by Morris water maze after 30 days of treatment. Then immunohistochemistry tests and Western blot technique were used to detect the content of RAS, P-MEK and P-ERK proteins in mouse brain. Results (1) The ability of learning and memory was significantly lower in model group than that of control group. The ability of learning and memory was significantly improved in treatment group than that in model group (P<0.05). (2) The contents of RAS, P-MEK and P-ERK in mouse brain were significantly lower in model group than those of control group, and these protein ex-pressions were significantly increased in treatment group than those in model group (P<0.01). Conclusion β-sheet break-er peptide H102 can activate ERK signal transduction pathway in brain of PAP double transgenic mice, increase PAS, P-MEK and P-ERK levels in nerve cells, and improve the ability of learning and memory in PAP mice.
