Study on Pathology and Neurotransmitters of Esophageal Sphincter after Radiofrequency Thermocoagulation in Dogs
10.3969/j.issn.0253-9896.2014.07.011
- VernacularTitle:狗食管下括约肌处射频控温热凝术后病理学及神经递质变化的研究
- Author:
Xiaodan ZHOU
;
Hongtao WANG
;
Yibin LIU
;
Zhonggao WANG
- Publication Type:Journal Article
- Keywords:
gastroesophageal reflux;
esophageal sphincter,lower;
choline O-acetyltransferase;
vasoactive intestinal peptide;
nitric oxide synthase;
dogs;
radiofrequency thermocoagulation;
lower esophageal sphincter;
choline acetyltransferase
- From:
Tianjin Medical Journal
2014;(7):664-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of radiofrequency thermocoagulation (RFT) on pathological features and the expressions of choline acetyltransferase (ChAT), vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) at lower esophageal sphincter (LES) in family dogs. Methods A total of 15 dogs were randomly divided into three groups. Sham group underwent gastroscopy and was fed for 3 months (n=5). Dogs were given RFT and were fed for 24 h after RFT (n=5, RFT+24 h group). Dogs were given RFT and were fed for 3 months after RFT (n=5, RFT+3m group). The pathological changes of LES were observed after HE staining in three groups. The expressions of ChAT, VIP and NOS were detected by immunohistochemical method in three groups. Results Results of HE staining showed nearly the same tissues in Sham group and control group. There were active inflammatory reaction and structural damage in RFT+24 h group. The chronic in-flammatory reaction and structural remodeling were found in RFT+3m group. Immunohistochemistry showed that ChAT was significantly increased in RFT+3m group compare than that of Sham group. Values of VIP and NOS were significantly de-creased in RFT+3m group compare than that of Sham group (P<0.01). Conclusion The thickness and increased pressure of LES were found after RFT,which also caused changes in neurotransmitters of local tissues in dogs.
