Effects of Cisplatin on Proliferation and Invasion of Colorectal Cancer Cells
10.3969/j.issn.0253-9896.2014.07.004
- VernacularTitle:顺铂对结直肠癌细胞增殖及侵袭能力的影响
- Author:
Yang XU
- Publication Type:Journal Article
- Keywords:
colorectal neoplasms;
cisplatin;
cell proliferation;
neoplasm invasiveness;
drug resistance,neoplasm;
SW480;
Na+-K+-ATPase
- From:
Tianjin Medical Journal
2014;(7):638-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480 cells were used as the research object, and untreated SW480 cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos-phate determination methods were used to detect proliferation, invasion and Na+-K+-ATPase activity expression level in SW480 cells. Results The physiological concentration of cisplatin (70 μmol/L) inhibited the proliferation of SW480 cells at 48 h. There was no significant difference in the inhibition rate at 48 h compared with 72 h and 96 h. Treatment with 70 μmol/L cisplatin for 48 h reduced the number of cells through Matrigel membrane matrix. The Na+-K+-ATPase activity was significantly increased in 35, 70 and 140 μmol/L cells after treatment with 17.5, 35, 70 and 140 μmol/L of cisplatin in SW480 cells for 48 h, and Na+-K+-ATPase reached the highest level at 70 μmol/L of cisplatin. Conclusion The decreased activity of Na+-K+-ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.
