Influence of CD146-siRNA on epithelial-mesenchymal transition in human ovarian cancer cell line A2780

VernacularTitle:CD146-siRNA对卵巢癌细胞A2780上皮-间质转化的影响
Author: Hui TIAN ; Jiajie WU
Publication Type:Journal Article
Keywords: Antigens,CD; RNA,small interfering; Epithelial cells/cytology; Biotransformation; Ovarian neoplasms/ pathology
From: Journal of Chinese Physician 2014;16(10):1374-1376
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of CD146-siRNA on epithelial-mesenchymal transition in human ovarian cancer cell line A2780.Methods The influence of loss of CD146 on the mRNA and protein expressions of E-cadherin,vimentin,and fibronectin was evaluated by real-time polymerase chain reaction (RT-PCR) and Western blotting.Results Compared to non-transfected (control group) and those transfected with RNAi-NC (RNAi negative control group),the mRNA and protein expressions of Ecadherin were significantly increased in A2780 cell lines transfected with RNAi-CD146.The loss of CD146 expression down-regulated the mRNA and protein expression of vimentin and fibronectin(P ＜ 0.05).Conclusions CD146-siRNA can suppress epithelial-mesenchymal transition in human ovarian cancer cell line A2780.