Establishment of double antibody sandwich ELISA for pro-gastrin releasing peptide and its application
- VernacularTitle:胃泌素释放肽前体双抗体夹心酶联免疫吸附法的建立及其应用
- Author:
Zhenyu CHU
;
Xiaolin ZHOU
;
Zhenwei XUE
;
Meiping CUI
;
Suqin LIN
;
Ruihua LI
- Publication Type:Journal Article
- Keywords:
Pro-gastrin releasing peptide;
Horseradish peroxidase;
Monoclonal antibody;
Enzyme-linked immune sorbent assay;
Tumor
- From:
Journal of Medical Postgraduates
2015;(1):70-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective The value of pro-gastrin releasing peptide ( PGRP) which is the tumor marker of small cell lung canc-er has become a hot topic in recent years .The research was to build a new enzyme-linked immune sorbent assay ( ELISA) method ai-ming at detecting the concentration of PGRP in patients′serum. Methods We utilized synthetic PGRP epitopes for the screening of the monoclonal antibodies , labeled the screened monoclonal antibodies with horseradish peroxidase by modified sodium iodide method , and then established double antibody sandwich ELISA which could be used to detect the serum concentrations of PGRP in cancer pa -tients. Results We successfully screened E 12 mAb which could be served as the coating antibody and ED 1 mAb as the labeled anti-body.The standard antibody density range of new ELISA was 33 pg/mL~1.7 ×104 pg/mL.The comparison experiments between our method and the commercially available ELISA kit showed no significant difference ( P>0.05).The specificity of our method was 50%, and the sensitivity was 100%, while IBL kit was 92.2% and 100% respectively. Conclusion New ELISA can be used to detect the serum PGRP concentration in patients with small cell lung cancer .
