Determination of specnuezhenide in Yishen Pills by high-performance liquid chromatography
- VernacularTitle:高效液相色谱法测定益肾丸中特女贞苷的含量
- Author:
Enzhong CUI
;
Anfu TANG
;
Wenya LIU
;
Hua SHENG
;
Hao TANG
;
Shudong WANG
- Publication Type:Journal Article
- Keywords:
Yishen Pills;
Specnuezhenide;
High-performance liquid chromatography
- From:
Journal of Medical Postgraduates
2015;(1):79-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective Former standards for Yishen Pills only identify angelica root , astragalus root and ligustrum lucidum by thin layer chromatography ( TLC) and there is no test for the content of effective components .The study was to improve the quality of Yishen Pills by perfecting the quality standards . Methods We determined the content of specnuezhenide in Yishen Pills by high-per-formance liquid chromatography(HPLC), with a mobile phase of MeOH-Water, a chromatography column of Agilent TC-C18(2) (4.6 × 250 mm, 5μm), a flow rate of 1.0 mL/min, a column temperature of 25℃, a detection wavelength of 224 nm, and a sample loop volume of 10μL. Results The linear relationship of specnuezhenide content was good in the range of 15.375μg/mL~246.000μg/mL.The relative standard deviation of precision experiment , stablity experiment and repeatablity experiment was 0.44%, 0.95%and 2.65%re-spectively.The average recovery was 99.60%.The qualified standard for specnuezhenide in Yishen Pills was ≥0.3 mg/g. Conclusion The method is simple , accurate and reliable , with good reproducibility , and it is applicable for the quality control of Yishen Pills .
