Effects of artesunate on high glucose-induced cell apoptosis, TNF-αand IL-8 expression in renal tubular epithelial cells
10.3969/j.issn.0253-9896.2015.01.006
- VernacularTitle:青蒿琥酯对高糖诱导的肾小管上皮细胞凋亡及TNF-α、IL-8表达的影响
- Author:
Shanshan JIANG
;
Yan LONG
;
Ke SU
;
Han NIE
;
Lili HUANG
;
Fan YANG
;
Zhengming LI
;
Jingqiong XUN
- Publication Type:Journal Article
- Keywords:
diabetic nephropathies;
apoptosis;
tumor necrosis factor-alpha;
interleukin-8;
artesunate;
renal tubular ep-ithelial cells
- From:
Tianjin Medical Journal
2015;(1):20-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of artesunate (Art) on cell apoptosis, tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) expression induced by high glucose in rat renal tubular epithelial cells (NRK-52E). Methods NRK-52E cells were cultured and divided into normal control group, high glucose group, high glucose with different concen?trations of Art (10 mg/L, 20 mg/L and 30 mg/L) groups, and high glucose with Ena (5 mg/L) group. MTT assay was used to de?tect the cell proliferation. The apoptotic rate was evaluated by flow cytometry with AnnexinV-FITC/PI double stains. The pro?tein levels of TNF-αand IL-8 in the cell culture supernatant were determined using ELISA. Results High glucose inhibit?ed NRK-52E proliferation, induced its apoptosis, and the expressions of TNF-αand IL-8 in the supernatant. Application of Art obviously abolished the effects of high glucose, and the effects of Art were showed in dose-dependent manner. Conclu?sion Art can suppress high glucose-stimulated cell apoptosis, enhance TNF-αand IL-8 expression in NRK-52E cells. The anti-inflammatory action and immune regulation of Art could be a novel approach of treating diabetic nephropathy.
