Polymorphisms of TNF-αgene and HSP70-2 gene in patients with acute pancreatitis
10.3969/j.issn.0253-9896.2015.01.015
- VernacularTitle:TNF-α和HSP70-2基因多态性与急性胰腺炎的相关性研究
- Author:
Jiaping HU
;
Jianxin ZHUANG
;
Yong LI
;
Yonghuan YU
;
Qinghong HU
;
Yinying LAI
;
Antao WU
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-alpha;
HSP70 heat-shock proteins;
pancreatitis;
genetic polymorphism;
HSP70-2;
polymorphism,restriction fragment length;
polymerase chain reaction
- From:
Tianjin Medical Journal
2015;(1):57-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of tumor necrosis factor (TNF)-α, heat shock protein (HSP)70-2 gene polymorphisms and susceptibility of acute pancreatitis(AP). Methods Using case-control method,The gene polymor?phism of TNF-α and HSP70-2 was detected by PCR-RLFP in 72 patients with AP and 71 healthy controls. Results There were no significant differences in proportion of TNF-αgenotype and alleles between AP and control groups (P>0.05). There were no significant differences in TNF-αgenotype and alleles between severe acute pancreatitis (SAP) and light acute pancreatitis (MAP) of AP group (P>0.05). There were no significant differences in white blood cell count, C-reactive pro?tein (CRP), amylase, three acyl glycerin and glucose between TNF-a and HSP70-2 gene of AA type and GA+GG type pa?tients (P>0.05). The HSP70-2 genotype GA+GG proportion was significantly higher in AP group than that of control group (69.4%vs 49.3%). The ratio of patients with G allele was significantly higher in AP group than that of control group(46.5%vs 31.7%). The ratio of patients with GA+GG type AP was significantly higher in SAP patients than that of MAP patients of AP group(81.0% vs 53.3%). There was no significant difference in G allele between SAP and MAP patients (P>0.05). Conclusion TNF-α polymorphisms is not associated with acute pancreatitis. There is an association between HSP70-2 polymorphisms and acute pancreatitis. Carrying the G allele increases the possibility of a severe acute pancreatitis ,which is one of the genetic susceptibility factors of severe acute pancreatitis.
