The Effects of UrsoIic Acid on CispIatin-Induced Expression of Transient Receptor PotentiaI VaniIIoid 1 in Mouse CochIea
10.3969/j.issn.1006-7299.2015.01.014
- VernacularTitle:熊果酸对顺铂致毒小鼠耳蜗瞬时感受器电位香草酸受体1表达的影响
- Author:
Yang DI
;
Li YU
;
Yuan TIAN
;
Yuhan LIN
;
Aimei WANG
- Publication Type:Journal Article
- Keywords:
Ursolic acid;
Cisplatin;
Cochlea;
Transient receptor potential vanilloid 1
- From:
Journal of Audiology and Speech Pathology
2015;(1):57-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ursolic acid (UA) on cisplatin (CDDP)-induced expres_sion of transient receptor potential vanilloid 1 (TRPV1) in mouse cochlea .Methods Sixty BALB/c mice were ran_domly divided into 4 groups (15 mice in each group) and received introperitoneal injection once daily for 5 days:Control group (normol saline) ,UA group (80 mg/kg/day) ,CDDP group (4 .5 mg/kg/day) ,and CDPP (4 .5 mg/kg/day) plus UA group (80 mg/kg/day) .The expression of TRPV1 in mouse cochlea was determined by immuno_histochemistry ,microscope image analysis and western blot ,and auditory thresholds were evaluated by auditory brainstem response (ABR) measurement .ResuIts The expression of cochlea TRPV1 and ABR threshold shift was significantly increased in the mice treated with CDDP (P< 0 .05) ,as compared with control mice .These effects were prevented by UA treatment (all P<0 .05) .Furthermore ,a linear relationship analysis revealed that the ex_pression of cochlea TRPV1 was significantly correlated with ABR threshold shift(|r|>0 .7 , P<0 .05) .ConcIusion UA effectively attenuated CDDP -induced ototoxicity and improved auditory function through inhibition of TR_PV1 .
