The Effect of Antisense miR-30d on Invasion and Migration in Breast Cancer Cells
10.3969/j.issn.0253-9896.2014.03.004
- VernacularTitle:反义miR-30d对乳腺癌细胞侵袭迁移能力的影响
- Author:
Ge CHEN
;
Ping LUO
;
Yu GONG
;
Yao ZHOU
;
Ruijun ZHAO
;
Chunwei XIE
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
carcinoma;
neoplasm invasiveness;
cell movement;
miR-30d;
antisense oligonucleotides
- From:
Tianjin Medical Journal
2014;(3):203-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR-30d in breast cancer tissues and demonstrate the regula-tive effects of miR-30d ASO on the invasion and migration of breast cancer cells in vitro. Methods The expression levels of miR-30d in 108 breast cancer tissues and their adjacent tissues were detected by real-time quantitative PCR method. Af-ter transfection with miR-30d ASO, the biological effects of miR-30d on in breast cancer cells was measured by transwell as-say and wound healing assay. The expressions of matrix metalloproteinase (MMP)-2 and MMP-9 were analyzed by Western blot assay. Results The expression level of miR-30d was found to be over-expressed in breast cancer tissues(P<0.05). Compared with control group and nonsense interference group, the miR-30d expression was significantly decreased in breast cancer cells(transfection with miR-30d ASO). Results of transwell and wound healing assay showed that the invasion and mi-gration ability decreased significantly after transfection with miR-30d ASO, and expressions of MMP-2 and MMP-9 were down-regulated (P<0.05). Conclusion miR-30d was over-expressed in human breast cancer. The invasion and migration of breast cancer cells can be effectively inhibited by decreasing the expression of miR-30d. miR-30d may become a new tar-get for the regulation of invasion and migration in breast cancer.
