Effects of Ellagic Acid on Melanogenesis and Melansome Transfer
10.3969/j.issn.0253-9896.2014.03.005
- VernacularTitle:鞣花酸抑制黑素细胞黑素合成及黑素传递的实验研究
- Author:
Dong LIU
;
Xiaogang PAN
;
Yalin LI
;
Junling ZHANG
;
Huijun MA
- Publication Type:Journal Article
- Keywords:
ellagic acid;
melanocytes;
monophenol monooxygenase;
keratinocytes
- From:
Tianjin Medical Journal
2014;(3):208-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ellagic acid on human epidermal melanocyte melanogenesis and melanin transfer, and the mechanism thereof. Methods The human melanocytes and and keratinocytes were co-cultured and purified. After passing the second generation, cells of 1∶10 ratio were inoculated into the small dish (3 cm × 3 cm). The changes of melanin content and tyrosinase activity in melanocytes were detected before and after intervention with ellagic acid (100, 10 and 1 mg/L) for 48 h. The melanin transfer in cultured cells was detected by flow cytometry method. The 10 nmol/L arbutin was used as the positive control. Results The tyrosinase activity was down-regulated by ellagic acid in a dose-dependent manner. The ellagic acid can reduce the melanin content except for the 1 mg/L of ellagic acid. The melanin transfer was also inhibited by ellagic acid in a dose-dependence manner. Conclusion Ellagic acid can be used for skin-whitening cosmetic and the depigmenting effect might be due to the down-regulation of melanogenesis and melanin transfer.
