Effects of Different Magnitudes of Mechanical Stretch on Human Intervertebral Disc Cells
10.3969/j.issn.0253-9896.2014.03.015
- VernacularTitle:周期性牵张应力对人椎间盘纤维环细胞合成和分解的影响
- Author:
Dongwei FAN
;
Zhongqiang CHEN
;
Zhaoqing GUO
;
Qiang QI
;
Weishi LI
- Publication Type:Journal Article
- Keywords:
fractures,stress;
intervertebral disk;
signal transduction;
intervertebral disc cells;
ADAMTS-4;
ERK
- From:
Tianjin Medical Journal
2014;(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different magnitudes of mechanical stress on human interverte-bral disc degeneration. Methods The human intervertebral disc cells were subjected to different magnitudes of mechanical stress (0, 6%, 12%, or 18%elongation) for 24 h using a Flexercell Strain Unit. The mRNA expressions of anabolic genes (col-lagen-1A1, collagen-2A1, aggrecan and versican) and catabolic genes (MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5) were examined by real-time PCR and Western blot methods. Results The expression levels of collagen-1A1 and collagen-2A1 were increased at 12%of mechanical stress, and collagen-2A1 was decreased at 18%of mechanical stress compared with those of control. The mRNA expressions of catabolic genes, MMP-13 and ADAMTS-5, were increased at 12%and 18%of mechanical stress than those of control. The mechanical stretch induced a magnitude-dependent increase in ADAMTS-4 synthesis, which was finely tuned by stretching-triggered activation of distinct mitogen-activated protein kinase cascades. Specifically, an ERK1/2 specific inhibitor, U0126, significantly inhibited the stretching-induced ADAMTS-4 expression, whereas the inhibitors of p38 and JNK, SP6000125 and SB203580, showed only slightly effect on the stretching-induced ADAMTS-4 expression. Conclusion The different magnitudes of mechanical stretch exhibited different effects on the bio-logical behavior of intervertebral disc cells, which profoundly affects the intervertebral disc degeneration.
