Bioeffects of canine myocardium under microbubble destruction via diversity of ultrasonic intension
10.3321/j.issn:1003-3289.2009.10.003
- VernacularTitle:不同强度超声破坏微泡对犬心肌组织的生物学效应
- Author:
Shigen ZHONG
;
Zhigang WANG
;
Zhiyu LING
;
Yuehui YIN
;
Qiao LI
;
Jie LUO
;
Xingsheng LI
- Publication Type:Journal Article
- Keywords:
Microbubble;
Bioeffect;
Myocardium;
Ultrasonography;
Intension
- From:
Chinese Journal of Medical Imaging Technology
2009;25(10):1738-1740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the bioeffects of canine myocardium under microbubble destruction via diversity of ultrasonic intension, in order to optimize ultrasonic intension for experiments. Methods Nine mongrel dogs were randomly divided into 3 groups. Ultrasound (1 MHz) in diversity of different intension (0.5 W/cm~2, 1.0 W/cm~2, 2.0 W/cm~2) was applied to expose canine myocardium after intravenous injection microbubbles of 2.0 ml. All the dogs were killed after being exposed for 5 min. The myocardium was harvested for HE staining and observed with transmission electron microscope for the tissue microstructures. Results The myocardium of hyperemia, disfiguration and necrosis wer observed in all groups. Myocardial edema but not hemorrhage appeared with 0.5 W/cm~2 , mild myocardial hemorrhage and slight inflammatory cell infiltration happened with 1.0 W/cm~2, whereas obvious hemorrhage and certain degree of inflammatory cell infiltration occurred with intension of 2.0 W/cm~2. With the augmentation of ultrasonic intension, myocardium trend to aggravate. Conclusion Ultrasound of diversity intension can induce different bioeffects of canine myocardium. Ultrasound mediated microbubble destruction with the intensity of 1.0-2.0 W/cm~2 can provoke a certain degree of inflammatory reaction with mild myocardial damage.
