The study of influence on cyclin and cyclin-dependent kinase inhibitor of Kasumi-1 cell treated with VPA
10.3760/cma.j.issn.1009-9921.2009.01.002
- VernacularTitle:丙戊酸钠对Kasumi-1细胞周期蛋白及周期蛋白依赖性激酶抑制剂的影响
- Author:
Lei ZHAO
;
Zhihua ZHANG
;
Cuimin ZHU
;
Wenliang TIAN
;
Changlai HAO
- Publication Type:Journal Article
- Keywords:
Leukemia,acute;
Cyclins;
Cyclin-dependent kinase inhibitor,Kasumi-1 cell line
- From:
Journal of Leukemia & Lymphoma
2009;18(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence on cyclin and cyclin-dependent kinase inhibitor of cell cycle of Kasumi-1 cell-derived from t(8;21)/AML treated with histone deacetylase(HDAC)inhibitor valproic acid(VPA).Methods RT-PCR assay was applied to detect mRNA expression of regulation factor of cell cycle of Kasumi-1 cell with VPA at different concentration and different time. Results VPA could down-regulate mRNA expression of cyclinD1,cyclinE1 and cyclinB1,and up-regulate mRNA expression of p21 WAF1/CIP1 with no obvious variation of mRNA level of p27KIP1. Condusion VPA could regulate cell cycle through the regulation of cyclins and cyclin-dependent kinase,arresting Kasumi-1 cell at G0/G1 phase.
