Preparative isolation and purification of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high-speed counter-current chromatography
10.1016/j.jpha.2012.03.004
- Author:
Mei YANG
;
Xinjun XU
;
Chunyan XIE
;
Jieyun HUANG
;
Zhisheng XIE
;
Depo YANG
- Publication Type:Journal Article
- Keywords:
Radix Eupatorii Chinensis;
High speed countercurrent chromatography (HSCCC);
12,13-dihydroxyeuparin
- From:
Journal of Pharmaceutical Analysis
2012;02(4):258-263
- CountryChina
- Language:Chinese
-
Abstract:
An efficient method for the isolation and purification of 12,13-dihydroxyeuparin from Radix Eupatorii Chinensis by high speed counter-current chromatography (HSCCC) was established in this paper.The ether extracts of Radix Eupatorii Chinensis were purified by HSCCC with a solvent system of hexyl hydride ethyl acetate-methanol-water (1∶2∶1∶2,v/v/v/v).The upper phase was used as the stationary phase and the lower phase as the mobile phase.About 8.4 mg of 12,13-dihydroxyeuparin was obtained from 200 mg of ether extracts from Radix Eupatorii Chinensis in one-step HSCCC separation,with the purity of 96.71％,as determined by HPLC.After methanolwater recrystallization,the purity of 12,13-dihydroxyeuparin reached 99.83％.Such a simple and effective method was fairly useful to prepare pure compound as reference substances for related study on Radix Eupatorii Chinensis.
