Rapid europium-sensitized fluorescent determination of ulifloxacin, the active metabolite of prulifloxacin, in human serum and urine
- Author:
Peng DONG
;
Na XU
;
Bo FU
;
Lei WANG
- Publication Type:Journal Article
- Keywords:
ulifloxacin;
prulifloxacin;
europium;
fluorescence;
sodium dodecylbenzene sulfonate
- From:
Journal of Pharmaceutical Analysis
2011;01(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
A new fluorescent method was developed based on the ulifloxacin-europium (Ⅲ)-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium (Ⅲ) and significantly enhanced the characteristic fluorescence of europium (Ⅲ).The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0× 10 8 -2.0× 10-6M with a detection limit of 2.0× 10-10 M (3σ).This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.
