The in vivo gene expression profile of the rAAV-2-hGM-CSF,rAAV-2-mGM-CSF vector modified bone marrow mesenchymal stem cell
10.3760/cma.j.issn.1009-9921.2008.02.004
- VernacularTitle:rAAV-2-hGM-CSF、rAAV-2mGM-CSF转导骨髓间充质干细胞的在体基因表达
- Author:
Zhengjun XIE
;
Fang YIN
;
Shuyun ZHOU
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cell;
rAAV-2;
Gene expression
- From:
Journal of Leukemia & Lymphoma
2008;17(2):91-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the in vivo gene expression profile of the recombinant adenoassociated-2 virus mediated human GM-CSF, mouse GM-CSF (rAAV-2-hGM-CSF, rAAV-2-mGM-CSE )vector modified bone marrow mesenchymal stem cell (BMSC). Methods We transduced the BMSC by rAAV-2-hGM-CSF, rAAV-2-mGM-CSF at the condition which have acquired before respectively, then transfused the in vitro gene modified BMSC after 12 days proliferation in vitro to 6 weeks old nude mice through tail vein,while the BMSC transfused in control group hadn' t been gene modified. 2, 4, 6, 8 weeks after transfusion, count the total white blood cells and detect the hGM-CSF, mGM-CSF concentration in nude mice serum at that time point. Results Nude mice serum hGM-CSF levels were 23.77, 25.32, 19.77, 15.25 ng/L at 2, 4, 6, 8 weeks after transfusion compare to 36.25 ng/L, the in vitro level before transfusion; mGM-CSF levels were 34.96, 34.84, 35.50, 32.93 ng/L at 2, 4, 6, 8 weeks after transfusion compare to 25.14 ng/L, the in vitro level before transfusion; at the same time point the nude mice serum mGM-CSF levels were 17.34,17.44, 14.68, 16.85 ng/L in control group, rAAV-2-mGM-CSF transduced BMSC made the nude mice white blood cell count increased, but no changes in nude mice white blood cell count at rAAV-2-hGM-CSFtransduced BMSC and control group. Conclusion BMSC as a gene therapy vehicle, it can be gene modified in vitro, then the gene modified BMSC could let the therapeutic gene to have therapeutic effects in vivo.
