Effects of the dosage of GM-CSF on the maturation stage of murine marrow-derived dendritic cells
10.3760/cma.j.issn.1009-9921.2010.03.009
- VernacularTitle:粒-巨噬细胞集落刺激因子剂量对小鼠骨髓源性树突状细胞成熟状态的影响
- Author:
Junjie YIN
;
Xinrong ZHAN
;
Yu WANG
;
Yakun KONG
- Publication Type:Journal Article
- Keywords:
Neoplasms,experimental;
Dendritic cells;
Granulocyte-macrophage colony-stimulating factor;
Cancer vaccine;
Immunotherapy
- From:
Journal of Leukemia & Lymphoma
2010;19(3):156-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different dosages of granulocyte-macrophage colony-stimulating factor (GM-CSF) on generating the routine bone marrow dendritic cells, and supply suitable dosage of GM-CSF on preparation of dendritic cell vaccines used for different purpose. Methods Using low (5 ng/ml) and conventional (20 ng/ml) and high dosage( 50 ng/ml ) of GM-CSF combined interleukin-4 ( IL-4 ) to induce murine bone marrow dendritic cells were performed, The phenotypes (CD_(11c), CD_(80), CD_(86)) and functional properties of the DC were compared by FACS analysis and MLR. Results The DC induced by low dosage of GM-CSF were immature DC, expressing low CD_(11c), CD_(80), and CD_(86). DC induced by conventional dosage were functional mature, expressing higher CD_(11c), CD_(80), CD_(86), which could induce allogeneic T lymphocyte responses. DC induced by the high dosage GM-CSF were the most phetotypicaUy and functional mature cells, expressing the highest CD_(11c), CD_(80) CD_(86), which could induce the strongest allogeneic T lymphocyte responses. Conclusion The dosages of GM-CSF affect the maturation stage of dendritic cells. Low dosage of GM-CSF generated immature dendritic cells, but conventional dosage and high dosage generated mature dendritic cells. DC generated through high dosage of GM-CSF were the most mature in phenotype and function.
