Role of PCR and FISH in diagnosis and differential diagnosis of primary lymphoma and lymphoma-like lesions of the uterine cervix
10.3760/cma.j.issn.1009-9921.2009.03.005
- VernacularTitle:PCR和FISH技术在子宫颈淋巴瘤与淋巴瘤样病变诊断和鉴别诊断中的作用
- Author:
Cuiling LIU
;
Min LI
;
Xin HUANG
;
Gehong DONG
;
Yan ZHANG
;
Zifen GAO
- Publication Type:Journal Article
- Keywords:
Lymphoma,non-Hodgkin;
Lymphoma,large-cell,diffuse;
Lymphoma-like lesion;
Immunohistochemistry;
Polymerase chain reaction;
In situ hybridization,fluorescence
- From:
Journal of Leukemia & Lymphoma
2009;18(3):139-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) in diagnosis and differential diagnosis of primary lymphoma and lymphoma-like lesion of the uterine cervix. Methods Three cases of primary cervical diffuse large B-cell lymphoma (DLBCL) and two cases of cervical lymphoma-like lesion were retrospectively studied by detecting their PCRIgH rearrangement and chromosomal break of IgH, bcl-6, bcl-2 and mye genes with FISH. Results PCRIgH gene rearrangement analysis demonstrated that the monoclonal rearrangement of IgH gene was occurred in 3 cases of cervical lymphoma and 1 case of lymphoma-like lesion, Interphase FISH analysis detected IgH gene break and bcl-6 gene break in all the three cases of cervical lymphoma, while no specific chromosomal break of IgH, and bel-6, bcl-2 and myc genes was detected in two cases of cervical lymphoma-like lesion.Conclusion PCR-IgH gene rearrangement is not specific to cervical B-cell lymphoma. Analysis of IgH gene break and bcl-6 gene break with interphase FISH is helpful to differential diagnosis between B-cell lymphoma and lymphoma-like lesion of the uterine cervix.
