Expression of CD95 and OX40L in Hepatocellular Carcinoma
10.3969/j.issn.0253-9896.2010.03.004
- VernacularTitle:肝细胞癌患者外周血CD95和OX40L的表达
- Author:
Keqiu LI
;
Yuliang WANG
;
Yalei WANG
;
Xuhong MIAO
;
Jian LI
;
Guang LI
- Publication Type:Journal Article
- Keywords:
carcinoma,hepatocellular;
antigens,CD95;
tumor necrosis factors;
RNA messenger;
flow cytometry polymerase chain reaction
- From:
Tianjin Medical Journal
2010;38(3):170-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the expression levels of CD95 and OX40 ligand(OX40L)messenger RNA(mRNA)in hepatocellular carcinoma(HCC),and their clinical values thereof.Methods:The lymphocytes of research objects were mixed with corresponding fluorescence labeled monoclonal antibody(McAb);CD95 expression by CD3 positive T ceils was quantitatively measured by dual color flow cytometry.The expression of OX40L mRNA was detected in peripheral blood mononuclear cells by fluorescence quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR).Results:The CD95 expression by CD3 positive T cells was significantly higher in patients with HCC(34±20)% compared with that of normal controls (20±7)%(t=2.960,P ＜ 0.01).The expression level of OX40L mRNA was significantly decreased in patients with HCC compared with that of normal controls(t=2.302,P ＜ 0.05).Conclusion:These results suggest that abnormal expressions of CD95 and OX40L play crucial roles in peripheral blood of HCC patients at the stage of human hepatocareinogenesis.
