Regulating Effects of Herb Cake-partitioned Moxibustion on the Expression of p53 and C-myc Protein in Rats with Ulcerative Colitis
- VernacularTitle:Regulating Effects of Herb Cake-partitioned Moxibustion on the Expression of p53 and C-myc Protein in Rats with Ulcerative Colitis
- Author:
Tianping ZHAO
;
Xiaomei WANG
;
Huirong LIU
;
Huangan WU
;
Shuang ZHOU
;
Xuejun CUI
- Publication Type:Journal Article
- Keywords:
Colitis,Ulcerative;
Indirect Moixbustion;
Genes,p53;
Proto-Oncogene Proteins C-myc
- From:
Journal of Acupuncture and Tuina Science
2010;08(3):154-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of herb cake-partitioned moxibustion treating ulcerative colitis (UC) from the relationship between expression of p53 and C-myc protein, and morbidity of UC. Methods: Rats model of UC was made with immune methods and local stimulation.Forty SD rats were divided into normal, model, herb cake-partitioned, and mild moxibustion group by a random number table, 10 rats in each group. Hanging moxibustion in the mild moxibustion group was applied to Tianshu (ST 25) and Qihai (CV 6) for 10 min. Two moxa cones of herb cake-partitioned moxibustion were applied to the same acupoints respectively, in the herb cake-partitioned moxibustion group. Expression of p53 and C-myc protein was measured with immuno- histochemistical method in the colonic tissue of rats with UC.Results: Postive area, strength,and the immunohistochemistry index of the expression of p53 and C-myc protein were found more in the model rats than those in the normal rats (P＜0.01), whereas less in the herb cake-partitioned moxibustion and mild moxibustion groups than those in the model group (P＜0.01). Conclusion: p53and C-myc play important roles in the morbidity and development of UC, and herb cake-partitioned moxibustion could regulate the expression of p53 and C-myc protein in the colonic tissue of UC rats.
