MR observation of labeling bone marrow mesenchymal stem cells in vitro and transplantation for treating brain injury in rats
- VernacularTitle:体外磁标记骨髓基质细胞移植治疗大鼠创伤性脑损伤的在体MRI观察
- Author:
Huali LI
;
Jingliang CHENG
;
Yong ZHANG
;
Juan WANG
- Publication Type:Journal Article
- Keywords:
Superparamagnetic iron oxide;
Bone marrow stromal cells;
Brain injuries;
Magnetic resonance imaging
- From:Chinese Journal of Medical Imaging Technology
2010;26(2):228-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To label rat bone marrow mesenchymal stem cells (BMSCs) with superparamagnetic iron oxide in vitro, and to monitor the survivorship and location of the labeled BMSCs in rat models of traumatic brain injury (TBI) with high field MR. Methods BMSCs were cultivated in vitro, and were labeled with SPIO. TBI models were built in the left hemisphere of the rats with Feeney's method. Then SPIO-labeled BMSCs were grafted stereotactically into the region nearby the contusion site 24 h later. The rats underwent MR examination 1 day, 3 days, 1 week and 3 weeks after implantation. Results Brown iron particles could be demonstrated in the SPIO-labeled BMSCs under inverted phase contrast microscope. Numerous intracytoplastic iron particles were stained with Prussian blue, and diffused distribution of iron particles could be seen in the intracytoplasm under electron microscope. At implanted sites low signal intensity could be observed on every sequence of MR examination, among which T2~*WI and SWI were better than other sequences, and SWI was the best. Conclusion MR is sensitive of tracking the survivorship and location of the labeled BMSCs, and SWI is the most sensitive sequence to detect the labeled cells.
