Chemiluminescence determination of melamine with LuminoI-K3Fe(CN)6 system
- Author:
Xiaoshuang TANG
;
Xiyan SHI
;
Yuhai TANG
;
Zhongjin YUE
;
Qiqi HE
- Publication Type:Journal Article
- Keywords:
chemiluminescence;
flow injection;
melamine;
urine;
plasma
- From:
Journal of Pharmaceutical Analysis
2011;01(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive chemiluminescence(CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe(CN)6 system in alkaline medium.The determination conditions were optimized.Under optimum conditions,the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10 97.0 × 10 6 g/mL with a correlation coefficient of 0.9992.The detection limits (3σ) were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample.The average recoveries of melamine were 102.6％ for urine sample and 95.1％ for plasma sample.Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method.The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.
