Influence of intra-bone marrow hematopoietic stem cell transplantation combined with infusion of donor lymphocytes upon the development of graft-versus-host disease and graft-versus-leukemia

VernacularTitle:髓腔内造血干细胞移植联合供者淋巴细胞输注对GVHD和GVL的影响
Author: Yanzhi BI ; Dongxiang ZENG ; Gang ZHAO ; Baoan CHEN
Publication Type:Journal Article
Keywords: Lymphocyte transfusion; Graftvshost disease; Graftvsleukemia
From: Journal of Leukemia & Lymphoma 2009;18(12):714-716
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of donor lymphocyte infusion(DU) by intra-bone marrow(IBM)or intravenous(Ⅳ)on the incidence of graft-versus-host disease(GVHD)and graft-versus-leukemia(GVL)after allogeneic peripheral hematopoietic stem cen transplantation(allo-PBSCT).Methods Female C57BL/6 mice as recipients received total body irradiation (TSr) Oil day 0,followed by injection of peripheral hematopoietic stem cells from mobilized male BALB/e with the granulocyte-colony stimulating factor(rhG-CSF),and DLI Was performed via Ⅳ or IBM.The extents of GVHD and GVL were compared in recipients received IBM-DLI with those received IV-DLI.The percentages of donor-defived cells and CD_4~+ CD_(25)~+ regulatory T cells(Treg) were detected by flow cytometry.Results It Was found that the frequency of GVHD and GVL were reduced in IBM-DLI compared with that of IV-DLI(P