The Effect of Tripterygium Wilfordii Polyglycosidium on Expression of Cytokines in Patients with Atopic Dermatitis
10.3969/j.issn.0253-9896.2009.07.016
- VernacularTitle:雷公藤多苷对特应性皮炎患者细胞因子表达的影响
- Author:
Yuhuan ZHANG
;
Yanling XING
;
Yanhua LIU
;
Xin LIAN
- Publication Type:Journal Article
- Keywords:
ripterygium wilfordii;
dermatitis,atopic;
interleukins;
interferon type Ⅱ;
RNA,messenger;
reverse transcriptase polymerase chain reaction
- From:
Tianjin Medical Journal
2009;37(7):574-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of tripterygium wilfordii polyglycosidium (TWP) on atopie dermatitis (AD) by detecting the expression of IL-2,IL-4,IFN-γ and IL-IO mRNA. Methods: RT-RCR was used to detect the mRNA of IL-2,IL-4, IFN-γ and IL-10 in peripheral blood mononuelear cells (PBMC)of AD cultured with different concentrations of TWP.ResuRs:(1)The expression of IL-2 was significantly different between the high concentration group and other groups(P＜0.05 or P＜0.01 ), and between the control group and the middle concentration group (P＜0.01). (2)The expression of IL-4 was significantly different between the three different concentration groups (P＜0.01 ). (3) The expression of IFN-γmRNA was significantly different between the high concentration group and the control, the low, the middle concentration groups (P＜0.05 or P＜0.01 ). (4)There was no significant difference in expression of IL-10 mRNA between the high concentration group and the middle concentration group (P＞0.05), there was significant difference among the other groups (P＜0.05 or P＜0.01).Conclusion: TWP can suppress the expression of IL-2, IL-4, IFN-γand IL-10 mRNA, but the low concentration of TWP can suppress the high-level expression of IL-4 and IL-10mRNA in vitro.
