Expression of Fas, bcl-2, Caspase 3 and Annexin-V in NB4 cell line treated with STI571 in vitro

VernacularTitle:伊马替尼对NB4细胞株表达Fas、Caspase-3、bcl-2和Annexin-V的影响
Author: Dewen ZHOU ; Xiujuan ZHAO ; Liangming MA ; Zhenhua QIAO
Publication Type:Journal Article
Keywords: STI571; NB4 cell line; Apoptosis
From: Journal of Leukemia & Lymphoma 2008;17(6):412-414
CountryChina
Language:Chinese
Abstract: Objective To explore molecular mechanisms of apoptosis induced by STI571 in human acute promyelocytie 1eukemia cell lines NB4.nethods The expression of Annexin-V,Fas,Caspase-3 and bcl-2 in NB4 cells were detected by FCM after the treatment of STI571 at(0.5,1.0,5.0 μmol/L)ranging for 24 h,48 h and 72 h.Results With the increasing dose of STI571,the expression of bcl-2,Caspase-3,Annexin-V,Fas in NB4 changed from(10.22±0.62)declining to (5.82±0.52),from(42.21±1.02)ascending to(52.35±0.83),from(25.A2±1.21)ascending to(37.84±0.63),from(18.21±0.81)to(21.41±1.02)respectively.With the dealing time increasing(24,48,72 h),the expression of bcl-2,Caspase-3,Annexin-V,Fas in NB4,changed from (5.81±0.52)declining to(2.51±0.43),from(52.31±0.83)ascending to(69.51±1.12),from(37.81±0.93)ascending to(78.62±0.83),from(23.41±0.73)to(26.53±1.02)respectively.Conclusion STI571 can enhance the apoptosis program to Ni4 in a time-dependence and dose-dependence manner,but no change to Fas was observed.