Effects of human bone marrow fibroblastoid stromal cell line HFCL on the biological characteristics of migrating and homing in multiple myeloma cell line RPMI8226
10.3760/cma.j.issn.1009-9921.2010.07.012
- VernacularTitle:人类骨髓成纤维样基质细胞对多发性骨髓瘤细胞迁移和归巢特性的影响
- Author:
Fanmei GE
;
Yanhui GAO
;
Qingxian BAI
- Publication Type:Journal Article
- Keywords:
Multiple myeloma;
Bone marrow;
Fibroblasts;
Receptors,CXCR4;
CD49d;
Integria alpha 4
- From:
Journal of Leukemia & Lymphoma
2010;19(7):418-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human bone marrow fibroblastoid stromal cell lines HFCL on the proliferation .migrating and homing of multiple myeloma cell lines RPM18226. Methods The co-culture system of RPMI8226 and HFCL was established through cell culture in vitro, growth curves were determined by trypan blue exclusion, cell cycle and expression of adhension molecule CD49d were detected by flow cytometry, and expression of CXCR4 gene was examined by RT-PCR. Results The proliferation of RPMI8226 cells in direct contact with HFCL cells group was inhibited strongerly than that in transwell group. The percentage of G1 phase cells of RPMI8226 cells in direct contact with HFCL group was higher than that of RPM 18226 in transwell group, while the percentage of S phase cells was lower. The expressions of CD49d and CXCR4 in RPMI8226 cells were down-regulated by HFCL cells, and that in transwell group was higher than that in direct contact with HFCL cells group. Conclusion Human bone marrow fibroblastoidss tromal cell HFCL can inhibit the proliferation and the expressions of CD49d and CXCR4 of multiple myeloma cell line RPMI8226, and can prevent multiple myeloma cells from migrating and homing.
