Influence of Semen Processing on Proportion of Aneuploid Sperm
10.3969/j.issn.0253-9896.2009.07.002
- VernacularTitle:精液处理方法对精子染色体非整倍体率的影响
- Author:
Yunshan ZHANG
;
Haining LUO
;
Qingxiu YANG
;
Ruoran MI
- Publication Type:Journal Article
- Keywords:
in situ hybridization,fluorescence;
spermatozoa;
centrifugation,density;
gradient aneuploidy;
chromosomes
- From:
Tianjin Medical Journal
2009;37(7):532-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the influence of semen processing methods on the proportion of the aneuploid sperm by detecting the sperm's chromosome X, Y, 18 using fluorescence in situ hybridization. Methods: Ten patients with mild ohgoasthenosperia, who were received ICSI treatment, were included in this study. Five semen samples of the patients were randomly selected to detect using Swim-up method (A group) and 5 using sperm-grad double-density centrifugation method (B group).Another 5 patients with mild oligo-asthenosperia were as control (C group). The CEPX / Y and CEP18 probe was used to detect the sperm of these 15 patients by fluorescence in situ hybridization. The proportion of aneuploid sperm was compared in three groups. Results: The sex chromosome aneuploid rates were (4.21±2.49)%, (3.24～1.49) % and (2.62±0.89) % in control, A and B groups. The rates of aneuploid chromosome 18 were (3.00±1.22)%, (2.00～1.22)% and (2.00±1.22)% in control, A and B groups. There were no significant differences in three groups (P＞0.05). Conclusion: The results showed that the methods of Swim-up and Sperm-Grad double-density gradient centrifugation could select sperms in motility potential and teratospermia,but not in normal chromosome sperms.
