Value of circulating galactomannan screening for early diagnosis of invasive aspergillosis in hematological patients

VernacularTitle:血清半乳甘露聚糖检测对血液病患者侵袭性曲霉菌感染早期诊断的价值
Author: Lili LI ; Jianmin WANG ; Weiping ZHANG ; Jun HOU ; Hui CHENG ; Jianmin YANG ; Xianmin SONG ; Li CHEN ; Shuqing Lü
Publication Type:Journal Article
Keywords: Enzyme immunoassay; Galactomannan; Invasive aspergillosis
From: Journal of Leukemia & Lymphoma 2011;20(7):418-421
CountryChina
Language:Chinese
Abstract: Objective To detect the value of serum galactomannan (GM) for diagnosis of invasive aspergillosis (IA) in hematological patients. Methods We prospectively evaluated the diagnostic value of twice-weekly screening for circulating Aspergillus fumigatus with sanditch enzyme linked immunosorbent assay. Results On the basis of the analysis of 472 serum samples from 113 episodes of 92 patients, the sensitivity, specificity, positive and negative predictive values of the sandwich ELISA for proven and probable IA cases were 83.3 %, 91.1 %, 78.9 % and 93.1 %, respectively, when samples with 2 consecutive positive results≥0.7 were used. Furthermore, GM antigenemia was detected before the onset of radiologic signs with a median of 7 days (range, 1-14 days), before isolation of Aspergillus with a median of 4 days (range, 1-7 days),and before anti-fungal therapy with a median of 6 days (range, 1-15 days). Conclusion The sandwich ELISA for GM detection is a reliable method for early diagnosis of IA in patients with haematological diseases.