Changes of RAGE mRNA and Protein Expression in Thyroid of Diabetic Mellitus Rats
10.3969/j.issn.0253-9896.2010.02.012
- VernacularTitle:糖尿病大鼠甲状腺组织RAGE mRNA和蛋白表达的变化
- Author:
Wei ZHAO
;
Mengyu ZHU
;
Hong ZHANG
;
Ning ZHENG
;
Xiaoming WU
;
Peihua FANG
- Publication Type:Journal Article
- Keywords:
diabetes mellitus,type 2;
thyroid gland;
insulin;
guanidines;
glyosylation end products,advancedrats,Wistar
- From:
Tianjin Medical Journal
2010;38(2):118-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of receptor for advanced glycation end products (RAGE)in the thyroid tissue of diabetes mellitus (DM)rats.Methods:The rat model of DM was established by high-lipid diet and vena caudalis injection of streptozocin.The rats of DM models were then randomly divided into three groups:DM group(n=17),insulin intervention group(n=16)and aminoguanidine intervention group(n=16).Nine normal rats were taken as controls.Twelve weeks after the establishment of DM model,rats were sacrificed and the thyroid tissue was taken to determine RAGE mRNA and the protein expression with the method of RT-PCR and Western Blot.Results:The levels of RAGE mRNA and protein expression were higher in the thyroid tissue of DM rats than those in controls (P＜0.05).The levels of RAGE mRNA and protein expression were lower in the thyroid tissue of insulin intervention group and aminoguanidine intervention group than thor of DM group (P＜0.05).Conclusion:The levels of RAGE mRNA and protein expression were up-regulated in the thyroid tissue of DM rats,which can be restrained by controlling blood glucose and blocking the formation of advanced glycation end products.
