The characteristics of epithelial-mesenchymal transition in human prostate cancer stem progenitor cells
10.3760/cma.j.issn.1006-9801.2015.04.004
- VernacularTitle:前列腺癌干/祖细胞上皮细胞-间充质细胞转换特点的实验研究
- Author:
Zhifang MA
;
Bin HAO
;
Rui TU
;
Nan WU
;
Shuhai ZHANG
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Stem cells;
Epithelial-Mesenchymal transition
- From:
Cancer Research and Clinic
2015;27(4):234-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To implore the characteristics of epithelial-mesenchymal transition(EMT) in human prostate cancer stem progenitor (S/P) cells isolated from LNCaP cell lines.Methods The S/P cells were obtained through florescence-activated cell sorting (FACS).Western blot and immunofluorescence assay were used to detect the S/P cells' EMT markers expression,such as E Cadherin,N Cadherin,Vimentin and Snail.Soft agar assay was used to detect the tumorigenesis ability of S/P cells.Cell migration assay was used to detect the migration ability of S/P cells.Results Compared with non S/P cells,the expressions of EMT markers,such as N Cadherin,Vimentin and Snail,were increased in S/P cells,while the expressions of epithelial marker and E Cadherin were decreased in S/P cells.After cultured for three weeks,S/P cells and non S/P cells both clonally grew.The colony numbers were (18.34±1.21) and (82.27±7.54),respectively (t =8.617,P =0.001).After cultured for 48 hours,the migration cells number was (25.33±5.13) in non S/P cells and (74.33±7.64) in S/P cells (t =7.953,P =O.001).Conclusions Human prostate cancer S/P cells isolated from LNCaP cell line have some characteristics of EMT,such as stronger tumorigenesis and migration ability,which could promote tumor invasion and metastasis.
