Human lung cancer cells grow on acellular rat lung matrix
10.3760/cma.j.issn.1006-9801.2014.11.002
- VernacularTitle:人肺癌H460细胞在脱细胞化鼠肺基质支架中的生长
- Author:
Haibo ZHU
;
Qian ZHANG
;
Xiuli WANG
;
Xiaoyu XU
;
Hong WANG
;
Ling WANG
- Publication Type:Journal Article
- Keywords:
Acellular matrix;
Lung neoplasms;
Rat;
Perfusion
- From:
Cancer Research and Clinic
2014;26(11):725-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of human lung canccr cells grown in a decellularized rat lung matrix by perfusion.Methods Lungs were harvested from adult SD rats.Native cells of rat lungs were removed using 0.1％ sodium dodecyl sulfate (SDS) and 1％ Triton X-100 by perfusion to create a decellularized rat lung matrix.After decellularization,Human lung cancer H460 cells were implauted into the decellularized rat lung matrix and grown in a customized bioreactor with perfusion of oxygenated media for 1-2 weeks.Results Decellularized rat lung matrix showed preservation of matrix architecture devoid of all rat cells.H460 cells could grow in the bioreactor.Conclusions Human lung cancer H460 cells can grow in a customized bioreactor on a decellularized rat lung matrix.This ex vivo model can be used potentially to gain a deeper understanding of the biologic processes involved in human lung cancer.
