Analyses of the restricted usage of TCR BV, BJ subfamily and their sequences in patients with breast cancer

VernacularTitle:乳腺癌患者TCR BV、BJ限制性取用及序列分析
Author: Jianbo ZHANG ; Xiaodong LYU ; Qingkai YU ; Yongping SONG ; Jun HU
Publication Type:Journal Article
Keywords: Breast neoplasms; Multi-polymerase chain reaction; Complementarity determining region
From: Cancer Research and Clinic 2014;26(9):605-607
CountryChina
Language:Chinese
Abstract: Objective To analyze the restricted usage of TCR BV,BJ subfamily and their sequences in patients with breast cancer.Methods The total RNA was extracted from tissues of lymph node metastasis,then reverse transcripted.The complete DNA sequence of TCR β-chain was amplified by multi-PCR.The recombinant plasmids were sequenced by using online TCR resources.Results 5 TCR β-chain CDS were obtained in two patients.TCR β-chain presented specific repertoire skewing in metastatic lymph node.There were selected usage of BV 2,BV 14,BV 29S1 of BV subfamily and BJ1S1,BJ2S2,BJ2S3,BJ2S5 of BJ subfamily.The clonal proliferation T cells had different CDR3 amino acid sequences.Conclusions There are selected usages of TCR BV,BJ subfamily in patients with breast cancer.The sequences of CDR3 are mostly different in TCR clone proliferation.