Low incidence of FANCF gene methylation in acute myeloid leukemia

VernacularTitle:FANCF基因甲基化在急性髓系白血病中罕见
Author: Fei CHEN ; Jiang WU ; Shangqin LIU
Publication Type:Journal Article
Keywords: Leukemia,myeloid,acute; Gene,FANCF; Methylation
From: Journal of Leukemia & Lymphoma 2012;21(6):335-337
CountryChina
Language:Chinese
Abstract: [Objective]To evaluate FANCF gene methylation status and its roles in the pathogenesis acute myeloid leukemia (AML).[Methods]Genomic DNA from primary 30 AML patients and 21 AML cell lines were subjected to FANCF methylation analysis by PCR based restriction enzyme digestion assay.FANCF protein expression was detected by Western blot.In addition,FANCF gene methylation status was further analyzed using bisulfate sequencing.[Results] No FANCF methylation was found in primary AML patients.One (4.76 ％) AML cell line contained FANCF methylation in the promoter region.The AML cell line was hypersensitive to MMC with absence of FANCF protein expression.[Conclusion] FANCF methylation is a rare event in AML,and does not contribute to the initiation of AML, but may contribute to the clonal transformation and cellular phenotype maintenance in some AML cell lines.