Construction of Packaging Plasmid pSNAV-hEndostatin-CMV-EGFP of AAV Encoding Human Endostatin cDNA
10.3969/j.issn.0253-9896.2009.07.003
- VernacularTitle:人血管内皮抑素腺相关病毒载体包装质粒0pSNAV-hEndostatin-CMV-EGFP的构建
- Author:
Junjie SUN
;
Liming YIN
;
Ruoran MI
- Publication Type:Journal Article
- Keywords:
endostatins adenoviridae plasmids genetic vectors
- From:
Tianjin Medical Journal
2009;37(7):535-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct the packaging plasmid pSNAV-hEndostatin-CMV-EGFP of adeno-associated virus vector (AAV) encoding human Endostatin (hEndostatin) cDNA. Methods: The hEndostatin eDNA obtained from plasmid pCD-sEndostatin was subcloned into the packaging plasmid pSNAV of AAV by molecular clone ways. The recombinant plasmid pSNAV-hEndostatin-CMV-EGFP was identified by PCR analysis, restriction enzymes analysis and sequencing analysis.Results: The recombinant pSNAV-hEndostatin-CMV-EGFP was correctly constructed and confirmed by PCR analysis, restriction enzymes analysis and sequencing analysis. Conclusion: The constructed AAV-hEndostatin packaging plasmid pSNAV-hEndostatin-CMV-EGFP could be the packaging plasmid of rAAV-hEndostatin-EGFP.
