Inhibition effect of small hairpin RNA on expression of Topo Ⅱ gene in K562/AS2 cell line resistant to arsenic trioxide

VernacularTitle:小干扰RNA逆转三氧化二砷耐药的白血病K562/AS2细胞Topo Ⅱ基因表达的实验研究
Author: Xuyan ZHANG ; Jiacai ZHUO ; Xin DU ; Ming LI ; Xiaomei TAO ; Dunyun SHI ; Jin LOU ; Qiongli ZHANG
Publication Type:Journal Article
Keywords: RNA,small hairpin; DNA topoisomerases,tope Ⅱ; Arsenates; K562/AS2
From: Journal of Leukemia & Lymphoma 2011;20(6):357-361
CountryChina
Language:Chinese
Abstract: Objective To investigate the reversal effect of Topo Ⅱα-shRNA and Topo Ⅱβ-shRNA on Topo Ⅱ gene in K562/AS2 cells. Methods Three pieces of Topo Ⅱα-shRNA and three pieces of Topo Ⅱβ-shRNA were designed,synthesized and transfected into K562/AS2 cells by liposome. Expression level of Topo Ⅱα and Topo Ⅱβ mRNA were determined by real time fluorescence quantitative PCR. The expressions of Topo Ⅱα and Topo Ⅱβ protein were assayed with flow cytometer. Results After treated with Topo Ⅱα-shRNA or Topo Ⅱβ-shRN A for 24 hours,the expression level of Topo Ⅱα mRNA and Topo Ⅱβ mRNA protein in K562/AS2 cells decreased at most (78.22±0.01) %,(31.17±1.27) % (P <0.05),and (57.36±0.01)%,(23.98±1.22) % (P <0.05) respectively. Conclusion The expression of Topo Ⅱ gene can be down-regulated after infected with Topo Ⅱ-shRNA in K562/ AS2 cell line.