Significance of micro RNA-21 expression in diffuse large B-cell lymphoma

VernacularTitle:微小RNA-21在弥漫大B细胞淋巴瘤中的表达及其临床意义
Author: Yanan GUO ; Jingqiong WANG ; Dan MENG ; Junming GUO ; Guoping ZHONG ; Wenying YU ; Zhibin GAO
Publication Type:Journal Article
Keywords: Lymphoma,large cell,diffuse; Micro RNA 21; Real-time polymerase chain reaction
From: Journal of Leukemia & Lymphoma 2012;21(5):269-272
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the expression of miR-21 in diffuse large B cell lymphoma (DLBCL)and normal lymph tissues and its potential relevance with clinicopathological characteristics.MethodsThe expression levels of miR-21 in 50 primary DLBCL and 12 normal lymph node tissue specimens were examined by TaqMan real-time polymerase chain reaction.The expression of bcl-2 and p53 was detected by immunohistochemistry staining. ResultsThe expression of miR-21 was significantly higher in tumor tissues than that in normal tissues, in GCB subtypes higher than in non-GCB subtypes. And it was negatively correlated with bcl-2(P=0.020),while positively correlated with p53(P=0.022). Up-regulated miR-21 expression was low in three years of survival rate. ConclusionMiR-21 may indicate a more aggressive phenotype and serve as a molecular prognostic marker in DLBCL. High-expression of miR-21 is a key feature that is correlated with cell proliferation in DLBCL.miR-21 may have some guiding significance in prognosis.bcl-2,p53 is possibly one of the targets of miR-21 in DLBCL.