Experimental study on triptolide reversing hypermethylation of APC in acute lymphoblastic leukemia cell line Jurkat

VernacularTitle:雷公藤内酯醇对急性T淋巴细胞白血病Jurkat细胞系APC基因去甲基化诱导表达机制的研究
Author: Xuemei WU ; Jianzhen SHEN ; Aifang YU ; Liping FAN ; Haiying FU ; Songfei SHEN ; Dansen WU
Publication Type:Journal Article
Keywords: Methylation; Gene,APC; Triptolide; Leukemia,T-cell,acute; Jurkat cells
From: Journal of Leukemia & Lymphoma 2009;18(6):327-330
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of triptolide(TPL) on the anti-oncogene-APC gene of acute lymphoblastic leukemia cell line Jurkat in vitro. Methods The effects of TPL on proliferation Jurkat cells were assayed by using cell culture, MTT. The effects of TPL on APC gene of Jurkat cells were analyzed by nested methylation specific PCR and RT-PCR. The effects of TPL on the proteinum expression of APC gene were detected by Western blotting analysis. Results Following the treatment of TPL, the cell proliferation rate was degraded as the treatment concentration increased and the culture time extended. The effects were dose and time-dependent. The 48 hour IC50 was 19.7 ng/ml. TPL can reverse hypermethylation of APC gene,and induce the expression of the mRNA and the proteinum. Conclusion Low dose TPL could depress the proliferation rate of Jurkat. The possible mechanism might be its reversing the hypermethylation of APC gene and activiting the expression of APC gene.