Detection of MLL gene rearrangement in acute leukemia and its clinical significance
10.3760/cma.j.issn.1009-9921.2008.04.006
- VernacularTitle:急性白血病MLL基因重排检测的临床意义
- Author:
Huimin GUO
;
Hongwei WANG
;
Wei LIU
;
Qiuxing LI
;
Yanhong QIN
;
Lei ZHU
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Leukemia,acute;
Rearrangement,MLL gene;
Nested RT-PCR
- From:
Journal of Leukemia & Lymphoma
2008;17(4):258-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the frequency of mixed-lineage leukemia(MEL)gene rearrangement and frequent types of fusion genes and the clinical characteristics in acute leukemia(AL)with MLL gene rearrangement.Methods MLL gene rearrangement was detected by multiplex nested RT-PCR in 109 cases of AL.Immunophenotypes were screened by flow cytometry and the chnical features of MLL gene rearranged were analyzed.Results MLL gene rearrangement were identified in 6.4%(7/109)of the patients,4 cases of AML,1 case of M2,1 case of M4,2 cases of M5,3 cases of.B-ALL.MLL-AF4,MLL-AF6,MLL-AF9,MLL-AF10.MLL-ENL were deleted by multiplex nested RT-PCR.Patients with MLL gene rearrangement presented higher initial white blood cell count(WBC)(P=0.019).Conclusion Multiplex nested RT-PCR is a fast and effective method to detect gene rearrangement of MLL in AL Patients with MLL gene rearrangement presented higher WBC and poor prognosis.
