Significance of WT1 gene expression in patients with myelodysplastic syndromes

VernacularTitle:WT1基因在骨髓增生异常综合征患者中的表达
Author: Jianlan LI ; Hongwei WANG ; Xiaohong LI ; Bo YANG ; Lei ZHU ; Yanhong QIN
Publication Type:Journal Article
Keywords: Myelodysplastic syndromes; Genes,wilms tumor; Reverse transcriptase polymerase reaction chain
From: Journal of Leukemia & Lymphoma 2009;18(2):104-106
CountryChina
Language:Chinese
Abstract: Objective To determine whether pattern of WT1 gene expression is a useful marker for establishing prognosis and tracking disease progression in patients with myelodysplastic syndromes (MDS). Methods RNA were extracted from bone marrow smears of 45 patients with MDS, the WT1 expression were tested by FQ-RT-PCR. Results The degree of WT1 expression was increased during disease progression of MDS. The WT1 expression level was increased more a logarithm grade when a RA patient developed into RAEB, the fluctuation of WT1 expression level was decreased a logarithm grade during RA. Conclusion WT1 gene is a useful marker for assessment in MDS patients. It pointed out may diagnosis RA if the WT1 expression level higher two logarithm grade than the mean of normal control for patients which didn't diagnose by morphology. It may suggest disease progress of MDS if the WT1 expression level increased more a logarithm grade recently. Further confirmation was needed because the case was limited.