Effects of SDF-1 on the chemotaxis of cord blood AC133+ cells
10.3760/cma.j.issn.1009-9921.2008.05.005
- VernacularTitle:基质细胞源性因子-1对脐血AC133+细胞迁移功能的影响
- Author:
Yanping MA
;
Lan MA
;
Shaodong ZHAO
;
Hua REN
;
Junlan WANG
;
Linhua YANG
- Publication Type:Journal Article
- Keywords:
Fetal blood;
Receptors,eytokine;
AC133+ cells;
Receptors CXCR4
- From:
Journal of Leukemia & Lymphoma
2008;17(5):331-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the stromal cell-derived factor on chemotaxis of cord blood ACl33+ cells.Methods The optimal SDF-1 concentration is detected in Transwell system.Migration was calculated from the number of cells found to have passed through an 8-um pore size polycarbonate membrane.Results The chemotactic rate of fresh cord blood AC133+ cells increases along with the concentration of SDF-1,however,it tends to be stable when the concentration of SDF-1 reaches 150 ng/ml.There is no difference in the chemotactic rate of cord blood AC133+ cells between the group with CXCR4-blocking antibody and the group without SDF-1.Conclusion Transwell Plate can simulate the phenomenon of cell crossing endothelium.The chemotactic rate of cord blood AC133+ cells increases progressively along with the concentration of SDF-1,however,the chemotactic rate tends to equability when SDF-1 reaches a certain concentration.The chemotactic rate does not have a marked change while continuing to increase the concentration of SDF-1.There is no difference in the chemotactic rate of cord blood AC133+ cells between the group using CXCR4-blocking antibody and the group without SDF-1.
