Application of BIOMED-2 system for the detection of IGH gene rearrangement in extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue lymphoma diagnosis
10.3760/cma.j.issn.1009-9921.2012.01.008
- VernacularTitle:BIOMED-2体系检测免疫球蛋白重链基因重排在黏膜相关淋巴组织结外边缘区B细胞淋巴瘤诊断中的应用
- Author:
Hongyan LIU
;
Liyan XUE
;
Jianming YING
;
Tian QIU
;
Shuangmei ZOU
;
Ning Lü
- Publication Type:Journal Article
- Keywords:
Gene rearrangement;
Lymphoma,B-cell,marginal zone;
BIOMED-2 system
- From:
Journal of Leukemia & Lymphoma
2012;21(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficiency of BIOMED-2 system in detecting IGH gene clonal rearrangement and application in diagnosis of extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) lymphoma. Methods Forty-five cases were collected, including 36 MALT lymphomas from different organs, 3 extranodal lymphoid tissue proliferative lesions and 6 severe gastritis associated with H pylori. DNA was extracted from the formalin fixed paraffin embedded blocks of these cases and the quality of DNA was assessed using the BIOMED-2 specimen mixed control primers. IGH gene clonal rearrangement was detected using IGH VH-JH primers. The sensitivity and specificity of BIOMED-2 PCR were analyzed.Results Adequate DNA (≥ 300 bp) was obtained in 31 of 45 samples (including 22 MALT lymphomas, 3 lymphoid tissue proliferative lesions and 6 severe gastritis), and the DNA from the other 14 samples was degraded seriously. 16 of 22 MALT lymphomas were positive of IGH clonal rearrangement with the sensitivity of 72.7 ％.In contrast,none of 6 severe gastritis was positive with the specificity of 100 ％ IGH and clonal rearrangement were detected in one of 3 lymphoid tissue proliferative lesions. Conclusion BIOMED-2 assay is an effient and reliable method for diagnosis and differential diagnosis of MALT lymphoma which is important for clinical practical value.
