Protective Effect of Gardenia Jasminoides Ellis on Lipid Peroxidation Injury of Pancreatic Subcellular Fraction Following Severe Acute Pancreatitis
10.3969/j.issn.0253-9896.2010.01.015
- VernacularTitle:重症急性胰腺炎对胰腺亚细胞器脂质过氧化损伤及栀子的影响
- Author:
Yanlei WANG
;
Yanwei WANG
;
Youling JING
;
Yibing ZHANG
;
Na SUN
;
Xiaojun WANG
- Publication Type:Journal Article
- Keywords:
pancreatitis;
acute disease;
mitochondria;
lysosomes lipid peroxides;
disease models;
animal;
GARDENIA JASMINOIDES;
rats;
Sprague-Dawley
- From:
Tianjin Medical Journal
2010;38(1):46-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore lipid peroxidation injuries of pancreatic mitochondria and lysosome following severe acute pancreatitis (SAP) and the protective effects of gardenia jasminoides ellis (GJE) thereof. Methods: SAP models were induced by retrograde injection of 1.5% sodium deoxycholate. The rats were randomly divided into three groups: sham group, SAP group and GJE group. The changes were measured in superoxide dismutase (SOD), malondialdehyde (MDA), membrane fluidity of mitochondria and lysosome in pancreas and succinic dehydrogenase (SDH) in mitochondria, acid phosphatase (ACP) in lysosome.The effects of GJE were also observed. Results: Compared with Sham group, the activity of SOD and membrane fluidity of mitochondria and lysosome were decreased in pancreas, and the content of MDA were increased; the releasing rate of ACP was significantly higher in lysosome; SDH in mitochondria was lower in SAP group(P < 0.01). The above-mentioned indexes were obviously ameliorated in GJE group than those in SAP group(P < 0.01). The correlation analysis indicated that MDA was negatively correlated with SOD, positively correlated with membrane fluidity (P < 0.01). Conclusion: GJE can alleviate lipid peroxidation by getting rid of oxygen free radicals of pancreatic subcellular fraction in the course of SAP, and protect the structure and function of pancreatic subcellular fraction.
