Effects of membrane-bound prostaglandin E2 synthase 1 inhibitor MK886 on cell cycle of leukemia HL-60/A cells

Yiqing LI; Songmei Yin; Liping MA; Danian Nie; Shuangfeng Xie; Xiuju Wang; Yudan WU

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Effects of membrane-bound prostaglandin E2 synthase 1 inhibitor MK886 on cell cycle of leukemia HL-60/A cells

VernacularTitle:膜结合型前列腺素E2合酶1抑制剂MK886对白血病HL-60/A细胞周期的影响
Author: Yiqing LI ; Songmei YIN ; Liping MA ; Danian NIE ; Shuangfeng XIE ; Xiuju WANG ; Yudan WU
Publication Type:Journal Article
Keywords: Leukemia,experimental; Cell cycle; MK886; Membrane-bound prostaglandin E2 synthase 1
From: Journal of Leukemia & Lymphoma 2012;21(9):513-516
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of membrane-bound prostaglandin E2 synthase 1 (mPGES-1) inhibitor MK886 on cell cycle of the human acute myeloid leukemia HL-60/A cells.Methods Flow cytometry,Western blot and ELISA were used to measure the difference of cell cycle,expression of cyclin D1, mPGES-1 among HL-60/A cells,MNC and HL-60 cells.The effect of MK886 on cell cycle,cyclin D1,mPGES-1,PGE2,P-Akt and c-myc of HL-60/A cells were observed.Results Compared with MNC and HL-60 cells,the expression of cyclin D1 and mPGES-1 were higher in HL-60/A cells,the percentage of G0-G1 phase was decreased [MNC (62.63±6.58) ％,HL-60 (38.86±2.25) ％,HL-60/A (30.53±2.15) ％]and S phase increased[MNC (12.18±4.43) ％,HL-60 (47.70±1.88）％,HL-60/A (57.56±1.54) ％](all P＜ 0.05).After treated with MK886,cell cycle was arrested in G0-G1 phase.The expression of mPGES-1,cyclin D1,P-Akt and c-myc and synthesis of PGE2 were decreased.Conclusion MK886 can arrest HL-60/A cell cycles in G0-G1 phase,which possibly through down-regulation of mPGES-1/PGE2,reduction cyclin D1,P-Akt and c-myc expression.