Proteomics Analysis of Component in Serum with Anti-asthma Activity Derived from Rats Treated by Acupuncture
10.1007/s11726-009-0326-y
- VernacularTitle:Proteomics Analysis of Component in Serum with Anti-asthma Activity Derived from Rats Treated by Acupuncture
- Author:
Yu WANG
;
Jianmei CUI
;
Shulan MA
;
Yanyan LIU
;
Leimiao YIN
;
Yongqing YANG
;
Xuejun CUI
- Publication Type:Journal Article
- Keywords:
Acupuncture Therapy;
Asthma;
Electrophoresis;
Proteome;
Rats
- From:
Journal of Acupuncture and Tuina Science
2009;7(6):326-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the differential proteins in the serum with anti-asthma activity of acupuncture to pinpoint the substance basis of anti-asthma activity of acupuncture by proteomics method. Methods: Two-dimensional electrophoresis of protein was employed to analyze the differential proteins in the serum with anti-asthma activity of acupuncture, and identify the differential proteins with mass spectrum technique. Results: In adrenalectomy (ADX)-asthma acupuncture group and asthma acupuncture group, two-dimensional electrophoresis showed that there was an obvious fuscous point (MW=44.1kD, PI=5.1), which was 60S ribosomal protein L13, or hypothetical protein FLJ32191 analyzed by mass spectrum technique, different from other groups. In ADX-asthma acupuncture group, there were two obviously fuscous points (MW=39.SkD, PI=4.9 and MW=39.SkD, PI=5.0), which were zinc finger protein 208, and thyroid hormone receptor-associated protein analyzed by mass spectrum technique, and two special proteins (MW=17.3kD, PI<4.5 and 16.9kD, PI<4.5), which were Cyclophilin A and zinc finger protein 91 analyzed by mass spectrum technique, different from other groups. Conclusion: There are multi-target effects regulating the whole body in acupuncture treating asthma relating to immunoloregulation, gene expression, and protein synthesization. The effects of acupuncture on the response proteins need further research.
