Expression of PKC θ and Th1/Th2,Tc1/Tc2 cells cytokines of patients with myelodysplastic syndromes and aplastic anemia
10.3760/cma.j.issn.1009-9921.2012.07.005
- VernacularTitle:骨髓增生异常综合征和再生障碍性贫血患者蛋白激酶Cθ及Th1/Th2、Tc1/Tc2类细胞因子的表达
- Author:
Xuejing YANG
;
Weihua ZHANG
;
Xiulian ZHANG
;
Wen SU
;
Runmei FAN
;
Xiaojing GE
- Publication Type:Journal Article
- Keywords:
Myelodysplastic syndromes;
Aplastic anemia;
PKC θ;
Th1/Th2;
Tc1/Tc2
- From:
Journal of Leukemia & Lymphoma
2012;21(7):401-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse expression levels of protein kinase C θ (PKC θ ) and its association with Th1/Th2,Tc1/Tc2 cytokines in peripheral blood mononuclear cells (PBMC) of patients with myelodysplastic syndromes and aplastic anemia (AA),and understand the pathogenesis of MDS and AA.Methods Fourteen patients with MDS-RA, fifteen patients with AA, and thirty health controls of PBMC were collected.mRNA expression levels of PKC θ were measured by RTQ-PCR,and the expression levels of Th1/Th2,Tc1/Tc2 cytokines were measured by the flow cytometry. Results The expression PKC θ mRNA (AA group:23.54±1.01,MDS group:23.76±1.58 ;health control group:27.12±1.12, P=0.004) and Th1and Tc1cytokines were statistically significant of among groups (all P＜0.05). Th2 and Tc2 cytokines were no statistical difference among groups (allP＞0.05). There were no statistical difference in the PBMC of PKC θ mRNA and Th1/Th2,Tc1/Tc2 cell cytokine (allP＞0.05) between MDS and AA.Conclusions The expression levels of PKCθmRNA and Th1,Tc1cells correlated cytokine in MDS and AA patients of PBMC are increases.
